In dHL60 cells, lectin blotting revealed decreased α2,3-linked and increased α2,6-linked sialylation. With differentiation, NEU1 protein levels decreased > 85%, PPCA and NEU2 proteins increased > 12.0-fold, and 3.0-fold, respectively, NEU3 remained unchanged, and NEU4 increased 1.7-fold by day 3, and then returned to baseline. In response to 3 distinct differentiating agents, dimethylformamide, dimethylsulfoxide, and retinoic acid, total NEU activity in differentiated HL60 (dHL60) cells was dramatically reduced compared to that of nondifferentiated cells. Granulocyte colony-stimulating factor protected against NEU2 protein upregulation, PMN surface desialylation and apoptosis. In proapoptotic PMNs, NEU2 protein expression increased > 30.0-fold. Mature human PMNs contained NEU activity and expressed NEU2, but not NEU1, the NEU1 chaperone, protective protein/cathepsin A(PPCA), NEU3, and NEU4 proteins. Using the chemical formula of the compound and the periodic table of elements, we can add up the atomic weights and calculate molecular weight of the substance.To gain insight into sialic acid biology and sialidase/neuraminidase (NEU) expression in mature human neutrophil (PMN)s, we studied NEU activity and expression in PMNs and the HL60 promyelocytic leukemic cell line, and changes that might occur in PMNs undergoing apoptosis and HL60 cells during their differentiation into PMN-like cells. The percentage by weight of any atom or group of atoms in a compound can be computed by dividing the total weight of the atom (or group of atoms) in the formula by the formula weight and multiplying by 100. If the formula used in calculating molar mass is the molecular formula, the formula weight computed is the molecular weight. The formula weight is simply the weight in atomic mass units of all the atoms in a given formula. When calculating molecular weight of a chemical compound, it tells us how many grams are in one mole of that substance.
For bulk stoichiometric calculations, we are usually determining molar mass, which may also be called standard atomic weight or average atomic mass.įinding molar mass starts with units of grams per mole (g/mol). This is not the same as molecular mass, which is the mass of a single molecule of well-defined isotopes.
#Na2co3 molar mass how to#
This is how to calculate molar mass (average molecular weight), which is based on isotropically weighted averages. The atomic weights used on this site come from NIST, the National Institute of Standards and Technology. This site explains how to find molar mass. The reason is that the molar mass of the substance affects the conversion. To complete this calculation, you have to know what substance you are trying to convert. These relative weights computed from the chemical equation are sometimes called equation weights.Ī common request on this site is to convert grams to moles. In chemistry, the formula weight is a quantity computed by multiplying the atomic weight (in atomic mass units) of each element in a chemical formula by the number of atoms of that element present in the formula, then adding all of these products together.įormula weights are especially useful in determining the relative weights of reagents and products in a chemical reaction.
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